CinderBio
CinderBio HTA-Proteases for Advanced Proteomics
CinderBio can help advance your proteomics workflows today
Gain additional sequence coverage
Identify proteins and sequence not seen with trypsin... (read more)
One-step 5-90 minute digests
One-hour turnaround from whole blood or raw samples... (read more)
Find markers your competitors can't
Identify novel biomarkers and unstable targets... (read more)
Increase throughput and speed
Rapid one-step digestion of immunoprecipitations... (read more)
De novo sequencing
Superior breadth and depth of mAbs sequence coverage... (read more)
About our enzymes
Volcanic enzymes for bottom-up proteomics
CinderBio's patented technologies allow us to produce new heat and acid stable HTA-enzymes for bottom-up proteomics and industrial applications that work faster and last longer than anything previously available. These unique enzymes are made from amazing microbes that thrive in hot volcanic waters around the globe.
These enzymes provide access to parts of the proteome unavailable with standard tryptic digest protocols. Sample preparation in as fast as 5 minutes[1] can be achieved allowing for efficiency in workflow turnaround times which make acute-care[2] clinical proteomics applications possible.
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Both Krakatoa and Vesuvius are semi-specific proteases (E, L, or F) which digest at high temperatures in acidic conditions (e.g., 80C, pH 3)
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Due to the heat and acid conditions, no chaotropes are required for digestion
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Alkylation is also not required as acid conditions ensure cysteines remain protonated
How our enzymes are different
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Desired bias for ribonucleoproteins, histones, and mitochondrial membrane proteins
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De novo sequencing of single proteins (e.g., antibodies)
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Fully automated sample prep system
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High sequence coverage using a simple protocol (e.g., histones)
Example applications
References:
[1] M.C. McCabe, V. Gejji, A. Barnebey, G. Suizdak, L.T. Hoang, T. Pham, K.Y. Larson, A.J. Saviola, S.M. Yannone, K.C. Hansen, From volcanoes to the bench: Advantages of novel hyperthermoacidic archaeal proteases for proteomics workflows, J Proteomics (2023) 104992
[2] Acute-care clinical proteomics applications are defined as requiring less than one hour total turn-around time.
Technologies
CinderBio is at the cutting edge of proteomics, allowing you to discover the world of unique proteins/protein sequence to advance precision medicine.
We unlock access to parts of the proteome unobserved with parallel standard methodologies. Our enzymes allow for the speed required to enable near real-time analysis to the bedside.
Let us show you how our unique coverage of proteins allows you to discover new biological insights and advance your science.
Our enzymes provide unique protein coverage
Protein coverage with trypsin alone vs. trypsin in combination with HTA-protease data.
Proteins with <20% coverage improvement are plotted in grey, proteins with >20% coverage improvement are colored by class: nucleic acid-binding (magenta), mitochondrial (yellow), chaperone (purple), nuclear (orange) and other (white). The dashed line indicates the 20% improvement boundary.
Sustainable Industrial Products and other Enzymes
Lipases, Proteases, Cellobiosidases, Cellulases, Xylanases, Xylobiosidases, Alpha-Amylases, Pullulanases
Cleaning and Sanitation for the food industry
Formulated all natural cleaning and sanitation products
Biofuels and side products
Formulated cellulases and xylanases for cellulosic degradation
Awards
CinderBio’s innovative technologies and business model have been recognized in national business plan competitions, competitive commercialization programs, and Federal research funding agencies:
